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The next morning, the overnight culture was subsequently diluted 100:1 in fresh LB medium supplemented with the same antibiotics and incubated for 23 h until reaching an optical density at 600 nm (OD 600 = 0.40.5)

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Reconstituting tirzepatide from lyophilized powder adds another variable, because your concentration depends on how much bacteriostatic water you add, which is something the tirzepatide reconstitution chart covers in detail

Chin J Aesthet Med

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