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In contrast, during chronic or infected wound states, the pH shifts to alkaline levels (pH 79) as a result of microbial activity 53,54

Of note was the large (8- to 13-fold) increase in the conversion of E1 into its hydroxylated and methylated forms compared to the 2- to 5-fold increase observed for most other metabolite ratios

200 L fractions were collected and 100 L of each fraction was transferred to a black microtiter plate to measure the fluorescence as described above

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