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Second, hypertension was ascertained by self-report of a doctors diagnosis, which may underestimate true prevalence due to undiagnosed cases or overestimate it if participants misunderstood or misrecollected what their doctor communicated

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Membranes were washed three times with Tris-buffered saline with 0.05% Tween and incubated in secondary antibodies for 1 h at room temperature