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5.1.3 Metabolic consequences Pro-inflammatory signals block insulin pathways (369)

Fully embryonated eggs were recovered from the top of the 23% and 25% sucrose layers using a 20-ml syringe with G20 2 needle attached, washed five times with HBSS-AB and pooled for further use

When the TSA method was used, the sections were treated with biotinylated goat anti-mouse/rabbit IgG (1:1000 dilution, Vector Laboratories, Burlingame, CA) for 1 h, and then incubated with HRP-conjugated-avidin for 30 min (1:100 dilution in TN buffer, TSA System) at room temperature