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We anesthetized mice with pentobarbital, and then the whole brains or spinal cords were collected following transcardial perfusion and overnight post-fixation with 4% paraformaldehyde

Insulin has pleiotropic effects in most cells for instance the brain (4)

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The fresh final product containing DA progenitors meeting quality-control criteria (Supplementary Table 1) was bilaterally transplanted into the putamen using a neurosurgical navigation system