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Description

Immunofluorescence microscopy can visualize tight junction proteins (ZO-1, occludin, claudins) to assess their localization and organization

These transporters, such as LAT1 (SLC7A5) and CAT-1 (SLC7A1), mediate the uptake of essential amino acids (e.g., leucine, arginine) that activate the mTORC1 signaling pathwaya central hub for integrating nutrient availability with insulin secretion and glucose metabolism