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No changes were noted in MAPK, another protein kinase, or glycogen synthase kinase-3beta

[DOI] [PMC free article] [PubMed] [Google Scholar] 70.Rinella M.E., Lazarus J.V., Ratziu V., Francque S.M., Sanyal A.J., Kanwal F., Romero D., Abdelmalek M.F., Anstee Q.M., Arab J.P., et al

0.04 g dry leaves were homogenized with 3% (w/v) sulfosalicylic acid and centrifuged at 3000 g for 10 min

Yeast two hybrid assay According to manufacturers instructions (ThermoFisher), pGBKT7 (bait) and pGADT7 (prey) vectors were digested with BamHI and EcoRI