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Matching of Tm values and optimization of annealing temperature were not required to ensure efficient amplification (see figure: Tolerance of different primer Tm values)

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J Neurochem 143:684696 Luangmonkong T, Suriguga S, Mutsaers HAM, Groothuis GMM, Olinga P, Boersema M (2018) Targeting oxidative stress for the treatment of liver fibrosis

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