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20 Herein, we address these knowledge gaps by employing human PCa specimens and in vivo and in vitro models of CRPC subtypes, combined with high-content quantitative imaging analyses, mechanistic studies, and therapeutic evaluations using organoid and xenograft systems

GSH Sepharose 4 FF maintained higher yield than the other columns at faster flowrates and was selected for all future experiments

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Ethical approval All animal-related procedures were conducted in accordance with the institutional ethical guidelines for the care and use of laboratory animals, following approval from the Institutional Animal Ethics Committee (IAEC) of Banasthali Vidyapith, Rajasthan, under approval number BV/IAEC/September2023/10

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