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2017) for protocol-specific concentrations

The concentrations of serum amylase, lipase, total cholesterol and triglycerides were quantified utilizing a commercially available kit with an automatic biochemical analyzer (Roche, Germany), adhering closely to the provided manufacturers guidelines

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Assays were run on an ABI 7300 Real-Time PCR System (Life Technologies), using a transparent 96-well plate and optical seals (4titude)

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