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Plasma, urine and muscle creatine was determined by the Jaffe reaction using a method adapted from Taussky ( Reference Taussky 27 ) and De Saedeleer & Marechal ( Reference De Saedeleer and Marechal 28 )

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Oxidative stress (OS) results in an imbalance between the systemic production of reactive oxygen species (ROS), also known as free radicals and antioxidant defence system, thereby, causing the damage of biomolecules and this leads to modification of proteins, lipids and amino acids as well as breakage of DNA strands