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Mice The 6 to 8-week wild type (WT) mice on a C57BL/6J background, Rosa26-CAG-LSL-Cas9-tdTomato (tdTomato fl/fl , T002249), MANF fl/fl (for KI), MANF fl/fl (for KO), Alb-Cre, and 6-week male nude mice (BALB/c background) were purchased from GemPharmatech Co., Ltd (Nanjing, China)