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Cells were fixed with 4% paraformaldehyde (PFA), permeabilized with 0.2% Triton X-100, and then postfixed with 1% PFA + 0.01% Tween-20

Under hypoxic or ER stress conditions, ATF4 upregulates BH3-only proteins such as HRK, PUMA , and NOXA , which influence mitochondrial integrity and autophagy