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A full matrix of evolutionary distances was calculated, and the phylogenetic tree representation was generated from this distance matrix, using the programmes FITCH and DRAWTREE, as part of the PHYLIP package 58

Immunofluorescence staining was performed on the above colon tissue with primary antibodies of anti-TLR2 (1:1000), anti-TLR4 (1:500), and anti-RAGE (1:500), and secondary antibody of Cy3 goat anti-mouse IgG (1: 200)

doi: 10.1016/j.advms.2019.12.008 166 AdamczewskaDSowikowska-HilczerJWalczak-JdrzejowskaR

Kiessling P, Lledo-Garcia R, Watanabe S, Langdon G, Tran D, Bari M, et al

Retinol and astaxanthin thus work in a complementary manner: while retinol dynamically promotes cell renewal, astaxanthin stabilizes and protects the results achieved, thereby both contributing to slow down skin sagging