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A large amount of evidence shows that the activation of the pyroptosis system in ALD includes the classical pathway of caspase-1 activation induced by the synergistic action of ATP, uric acid and ROS, and the non-classical pathway of caspase-4/5/11 activation triggered by lipopolysaccharide (LPS) (Liu et al., 2022

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If youre using a more normal concentration of pH buffer, say 20mM Tris pH 8, then adding 10-20mM glutathione will make your elution buffer more acidic