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Methods Cell Culture All cell lines were cultured in high-glucose DMEM (4.5 g/l glucose, 110mM pyruvate

10,11 In clinical practice, many patients express concerns about pore visibility and seek effective treatments

Therefore, we hypothesized that the excessive expression of GJs post-APAP exposure in hepsin / mice could be a key factor contributing to the increased severity of liver damage observed following APAP administration

It is crucial to inhibit both apoptotic and necrotic pathways simultaneously, given that both apoptotic and necrotic signalling pathways are simultaneously involved in the pathogenesis of diseases such as ischaemia/reperfusion injury 17,18,19,20

It targets nerve signals rather than immune or inflammatory processes