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After the endocytic uptake of cargo with CPPs, transepithelial transport can be accomplished via transcytosis, avoiding lysosomal degradation ( Among the passage routes of drugs via nasal mucosa, large molecules like peptides, proteins, and nucleic acids are often reported to cross the nasal epithelium following the transcellular pathway or endocytosis (Ozsoy et al., 2009)

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[11] Tube-formation assays: Endothelial cells can form short-lived, tube-like networks on a laboratory gel

After washing, DAB reagent was added to the slices and incubated at room temperature for 3 min