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At mid-log the ODs of the bacteria were normalized to OD1, washed twice in sterile PBS and resuspended in sterile PBS

Potential dangers are allergic reactions, hormonal disruptions, and unverified long-term implications

In any case, the dried samples should be ready before estimation, tests processing utilizing a basic planetary ball factory (LZQM0.4L, Shicheng Desert spring Mineral Gear Assembling Co., Ltd.) in which ball factory of treated steel of 0.1 cm breadth put in processing measure with tests for 1 h at 1500 rpm [26]