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Two inhibitors of cysteine proteases (cystatins 39,40 ) and two -2-macroglobulin homologues (I39) were also identified, as were three inhibitor of apoptosis proteins (I32), one of which is highly expressed in adults, where it may function to regulate one or more of the four schistosome caspases

The release of fluorogenic AMC from the substrate was measured by Infinite M200 fluorometer (Tecan, Mnnedorf, Switzerland) at 24 C in a 30 min kinetic cycle (2-min intervals)