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Researchers have documented that each freeze-thaw cycle damages peptide chains, and pre-splitting eliminates the cumulative loss that comes from thawing one bulk vial multiple times

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Then a solution of the intermediate 2 (5 mmol, 1.08 g) in DMF (13 mL) was added dropwise into the above mixture while keeping the reaction temperature (0 C) basically the same

The chemical reactions which occur in the bisulfite sequencing protocol result in altering and degrading the DNA, providing only short strands for analysis, and therefore not being used for long-read sequencing [71]

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