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Briefly, frozen liver aliquots were homogenized in Serine Assay Buffer in a Potter-Elvehjem homogenizer and centrifuged at 15000 g, for 10 min at 4C

The citric acid cycle involves three NAD+-dependent dehydrogenase reactions: isocitrate dehydrogenase, -ketoglutarate dehydrogenase, and malate dehydrogenase, each generating NADH while releasing carbon dioxide and regenerating oxaloacetate for continued cycle operation

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The N-terminal cytoplasmic tail (N-tail) of GNPT is known to bind directly to the / and / subunits of COPI, and this interaction is critical for the Golgi localization of GNPT 21

The molecular mechanism underlying the relationship between OXPHOS and PDAC progression still requires in-depth study with bright prospects (Text Box S2)