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This measurement is converted to a percentage form, such as 0.10 percent

Membrane was then blotted with antibodies to either IR (Cell Signaling Technology, Danvers, MA, Cat#3025), IGF-1R (Cell Signaling Technology, Cat#9750) or -actin (Cell Signaling Technology, Cat#4970) for 1 h at room temperature, up to overnight at 4 C

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Figure 1A shows the plot generated by the D 2 P 2 platform: this analysis showed that the mature IGF-1 is mostly ordered, while all E-domains were predicted to contain disordered residues