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The samples were subsequently reduced with 4 mM DTT for 30 min at room temperature with shaking at 1,000 rpm, followed by alkylation with 10 mM iodoacetamide for 45 min in the dark at room temperature while shaking at 1,000 rpm

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They also trap and kill pathogens in an extruded web of DNA coated with antimicrobial peptides and cytotoxic histones, termed extracellular traps [5]