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251 Furthermore, both obesity and aging drive the expansion of double-negative (DN) B cells (CD27IgD), which display a proinflammatory phenotype marked by autoimmune antibody secretion and upregulated expression of activation markers (e.g., CD11c and T-bet)

Subsequently, the colonies were treated with a 4% paraformaldehyde (PFA, Servicebio, Wuhan, China) solution and then stained using crystal violet (Servicebio)