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Purified His-tagged VAMP8 and STX17 (1 mg/mL in 20 mM HEPES pH 7.4, 150 mM NaCl) were incubated with GST/GST-G3BP1-bound beads (10 g bait protein/mg beads) in interaction buffer (20 mM Tris-HCl pH 7.5, 350 mM NaCl, 1% Triton-x100, 1 mM EDTA) for 12 h at 4 C with gentle rotation

Muthukumaraswamy SD, Carhart-Harris RL, Moran RJ, Brookes MJ, Williams TM, Errtizoe D, et al

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In contrast, biocatalytic synthesis readily afforded the targeted compounds with isolated yields between 28% and 54% (Fig

360 MSU crystals cause cellular damage and mediate inflammatory responses, such as prostaglandin, bradykinin, IL-1, IL-6, and TNF-a