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HCT116 and HuH7 cells were infected with the lentivirus, and a stable cell line was selected by maintaining the cells in medium containing 10 g ml 1 blasticidin (Invitrogen) for 1 week

In the United States alone, the annual cost of PD exceeds $52 billion, including direct medical expenses and indirect costs such as lost productivity and caregiver burden (Ola et al., 2022

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There are two main distinct categories of cell death, namely accidental cell death (ACD) and regulated cell death (RCD) (Tang et al., 2019)

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