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Eye pressure may also be evaluated in different ways such as with a hand-held electronic device or a gentle puff of air to indent the eye

The fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 5 min and stained with 1 M C11-BODIPY for 30 min at 37 C The green (oxidized) and red (reduced) fluorescence images were simultaneously acquired using a Zeiss LSM 880 confocal microscope

Ophthalmology 116 SchollH

Targeting oxidative stress in ulcerative colitis Proinflammatory cytokines like NOS and ROS are more involved in the genesis and progression of UC (44)

The bacteria were centrifuged (4000 g, 10 min) and washed twice with sterile phosphate-buffered saline (PBS) solution, and finally resuspended in sterile PBS before use