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Membranes were then blocked in Starting Block T20 solution (Thermo Fisher Scientific) at room temperature for 1 hour, washed 3 times with PBS with Tween-20 (PBST), and incubated with an appropriate dilution of the primary antibody (all antibodies were used at a dilution of 1:1,000 except for GAPDH and -actin, which were used at dilutions of 1:5,000) in Starting Block T20 solution overnight at 4C

These groups were kept on Urochloa brizantha cv

Nature 293: 411-412

Cell Host Microbe 24 , 703716.e3 (2018)

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