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It explored the mechanisms of action of GLP-1RAs and their therapeutic potential in a wide array of diseases, such as diabetes mellitus, providing new insights into metabolic disease management

The protein was purified with Strep-affinity chromatography (for more details see supplementary figure 2), the tag was removed by TEV-cleavage and the protein was dialyzed into buffer W (100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH 8.0)

doi: 10.1055/s-2007-978596

Determination of intracellular reactive oxygen species (ROS) levels Total intracellular ROS levels were determined using a ROS assay kit (cat