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doi: 10.1002/ptr.4898 42 GongYLuoSFanPJinSZhuHDengTet al

untransformed cells

Another study that focused on identifying ALS biomarkers found that the deglutathionylating enzyme human GSTO1 was significantly reduced in peripheral blood mononuclear cells and spinal cord cells of sALS patients 147

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Given the potential impact of these factors on the quantification of GSH, here we examined if the type of anticoagulant used during sample collection (heparin or EDTA), deproteinization of plasma prior to storage (yes or no), storage temperature (80 o C or 4 o C), and processing time (duration of time between collection of whole blood and deproteinization of plasma) impact levels of free GSH and GSSG, in plasma