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The fundamental principle of the pull-down assay service involves immobilizing a tagged fusion protein onto an affinity resin to serve as the "bait protein." When the tagged fusion protein is incubated with the target protein, tissue, or cell lysate, the proteins that specifically interact with the bait protein are captured

S2CID 6962119

Rosenwasser AM, Turek FW

In the dsbA mutant, the presence of monothiols had no significant impact, while cystine supplementation caused a drastically increased re-oxidation state and rate, similar to WT (Fig