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The expression of SELENOW is correlated with selenium status and appears to be sensitive to low-selenium supply (19, 20)

The resulting supernatant was used for biochemical analysis using a Shimadzu UVvisible spectrophotometer

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To achieve quantification and external validation, high-resolution imaging should be paired with mechanical measurements to resolve skin-surface microrelief trajectories and cellECM interaction forces, with standardized QC readouts across barrier function, secretome, and mechanics 131,134