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After incubation, the batches were centrifuged at 4000 g for 25 minutes and the pellets were re-suspended in lysis buffer (200 mM NaCl, 50 mM Tris-HCl, 5 mM EDTA, 2 mM benzamidine, 1 mM phenylmethylsulfonylfluoride and 0.5 g/l lysozyme, pH 7.8)

Mitochondria-targeted therapies, including antioxidants [such as, coenzyme Q10, N-acetylcysteine (NAC) and -lipoic acid] and mitochondrial nutritional supplements, have emerged as promising alternatives aimed at mitigating oxidative stress and restoring mitochondrial function

We advise using the area around the belly button if it has some fat