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J Feline Med Surg 17(7), 606-613

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If the beta hCG level is not doubling as we expect it to do early on, it is generally not a good sign (it could be indicative of a biochemical or clinical pregnancy loss or ectopic pregnancy) and necessitates closer monitoring and possibly further diagnostic procedures

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Quick answer Documented research protocols reconstitute the 5000 iu HCG vial with 2 mL of bacteriostatic water, and the literature describes drawing 500 iu per dose from the prepared solution