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Acknowledgments We are very grateful to Dr Shusuke Kuge (University of Tokyo) for providing the plasmid pRS cup cp-GFP-YAP1 and Dr Marek Zagulski (Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw) for his help in the conversion of this construct for use in our strains

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Protocol four: growth hormone secretagogue foundation Goal: Elevate endogenous GH and IGF-1 to create a favorable systemic environment for bone healing