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75 These techniques, however, could not detect DNA interstrand cross-linking in mammalian cells exposed to therapeutic levels of alkylating agents in vitro or in tissues after in vivo drug administration

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Insights from these assays mitigate the risk of late-stage conflicting data, drug candidates being wrongly abandoned as toxic or mistakenly classified as safe

In the context of SSMD, ensuring that scientists have identified and can agree on the appropriate ortholog to human GPX4 for manipulation is critical, and as a selenoprotein GPX4 presents further challenges across other species

FIGURE 5 Antioxidant Treatment Rescues the Aggravated Spiral Ganglion Neurons Loss and Apoptosis Induced by Glutathione Peroxidase 1 Deficiency After Peroxynitrite Injury To explore whether GPX1 plays a protective role in peroxynitrite-induced SGNs damage by reducing intracellular ROS, we conducted a rescue experiment by using the antioxidant N-acetylcysteine (NAC)