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0.04 % p -nitrophenylphosphate (Sigma-Aldrich, CAS-333338-18-4) were added and samples were incubated until the solution turned visibly yellow, before the reaction was stopped by adding 200 L 1 M K 2 HPO 4

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1 H NMR (300 MHz, DMSO) 8.23 (d, J = 7.4 Hz, 1H), 8.12 (d, J = 8.2 Hz, 1H), 7.41 (d, J = 6.6 Hz, 9H), 7.23 (s, 1H), 7.12 7.04 (m, 6H), 6.78 (d, J = 5.4 Hz, 1H), 6.67 (d, J = 11.0 Hz, 1H), 4.56 (d, J = 6.6 Hz, 1H), 4.14 (q, J = 7.4 Hz, 1H), 3.60 (s, 3H), 3.51 (d, J = 8.0 Hz, 2H), 2.78 (dq, J = 32.2, 7.5 Hz, 4H), 1.36 (d, J = 2.4 Hz, 24H)