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Immediately read the absorbance at 412 nm in a microplate reader and take measurements every 30 s for 2 min (5 readings)

10.1016/j.neuint.2017.04.006 128 OttoliniD.CaliT.NegroA.BriniM

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Extracellular vesicle (EV) protein and miRNA profiles in COPD BAL show significant alterations, particularly increased expression of inflammation-associated proteins (e.g., neutrophil degranulation markers) (172),Notably, BAL from COPD patients induces stronger neutrophil chemotaxis, potentially linked to disease severity

The diagnostic hierarchy operates through multiple analytical approaches