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Levofloxacin persisters (levopersisters) were quantified as CFU/mL, and minimum inhibitory concentrations (MICs) were assessed using the broth microdilution method, following the Clinical and Laboratory Standards Institute (CLSI) guidelines [21]

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The fading of the observed initial growth inhibition is likely due to the lack of additional T-cell co-stimulation, the limited lifespan of the co-inoculated PBMCs and the absence of T-cell influx into the developing tumours, as would normally occur in an immunocompetent host

Interestingly, the screening revealed the existence of an allosteric pocket close to the NADPH binding site, named the doorstop pocket since ligands binding at this site interfere with TR activity by hampering the opening movement needed to allow cofactor binding

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