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The signal sequence directs secretion of the protein, for example, from a eukaryotic host into which the expression vector is transformed, and the signal sequence is subsequently or simultaneously cleaved

doi: 10.1007/s00520-010-1018-3 34 KautioALHaanpaaMLeminenAKalsoEKautiainenHSaartoT

10.1177/0363546508331137 13 Dietrich-ZagonelF.AspenbergP.EliassonP

Similar to the postfusion arrangement, this position would most probably clash with the N-terminal loop region of the DIII helix observed in our structure (Fig