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Thus, newly formed AuNPs with the increased size effectively prevent the exocytosis of nanoparticles and reduces their backflow to the bloodstream

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After incubation, the culture medium was collected, centrifuged at 2,000 g for 10 min to remove cellular debris, and the supernatant was carefully harvested for subsequent cytokine quantification using enzyme-linked immunosorbent assays (ELISA) kits: Mouse TNF alpha SimpleStep ELISA Kit (ab208348

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Intriguingly, in 2005, Mandal et al