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Expired or improperly stored MOTS-c loses its three-dimensional peptide structure through denaturation, rendering it inactiveit wont bind to AMPK receptors effectively, meaning youre injecting an inert solution with no metabolic benefit

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In biochemical research systems, it modulates metabolic signaling by interfering with NNMT catalytic activity, thereby influencing levels of methylated nicotinamide derivatives and related metabolites

doi: 10.1056/NEJMoa1506348 10 HariPRajRVOlteanuH