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After cooling to 0 C, -BriBr (1.54 mL) was added in CH2Cl2 (10 mL)

CXCL-2 forward, 5-TCCAGGTCAGTTAGCCTTGC-3 (SEQ ID NO:21)

It was initially thought that the presence of a particular histidine residue at the 63rd position with a pK a of around 6.4 probably serves a critical role in mediating this pH dependent switch of the protein

Acta Pedol Sin 61:348360 Zhang X, Zhou R, Teng L, Chen H, Li M, Wang L, Zhran M, Cao F (2024b) Genotypic variation in grain cadmium concentration in wheat: insights into soil pollution, agronomic characteristics, and rhizosphere microbial communities

The mice were exposed to a standard 12-h lightdark cycle