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The prepared tissue sections and cells were blocked with 5% BSA for 30 min and then incubated at 4C overnight with the following primary antibodies: MAP-2 (1:500, Abcam, United States), Ace-tubulin (1:500, Cell Signaling Technologies, United States), Tyr-tubulin (1:500, Sigma Aldrich, United States), GRP78 (1:1000, Abcam, United States), and MBP (1:500, Cell Signaling Technologies, United States)