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1B) In addition, Cabral et al

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Neurodevelopmental problems at 18 months among children exposed to paracetamol in utero: a propensity score matched cohort study

Intracellular staining for flow cytometry Experiments involving human blood samples were carried out in accordance with the approved guidelines and regulations according to the Declaration of Helsinki

Next, the mixture was centrifuged and the supernatant was used to determine the protein concentration with a BCA kit (Beyotime), and protein samples were then mixed with 5 sodium dodecyl sulfate (SDS) loading buffer (Beyotime) prior to denaturation in the boiling water bath for 5 min