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Ticks, as disease vectors, have evolved specific mechanisms to defend themselves against potential harm from pathogens

[DOI] [PubMed] [Google Scholar] [38].Maruyama E, Kojima K, Higashi T, Sakamoto Y

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However, reports using a variety of protocols used to generate tol-DC in vitro 60 indicated that metabolic plasticity and the heterogeneous nature associated with inherent epigenetic and transcriptional reprogramming is a cofounding factor in precise understanding of tol-DC 38,61

Then, 5% nonfat skim milk in PBS containing 0.05% Tween-20 was used for blocking