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Its best to start early, ideally when fine lines first become noticeable with facial expressions

Oxidative stress impairs oocyte quality and melatonin protects oocytes from free radical damage and improves fertilization rate

The samples were rinsed with PBS and fixed 4% formaldehyde solution for 30 min, followed by permeation with 0.1% TritonX-100 for 1 min

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