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Allu, A

Gel lanes were cut in several pieces, and proteins were reduced, alkylated, and digested by overnight incubation with trypsin (bovine sequencing grade, Roche Applied Science, Monza, IT) according to published protocols ( 2.10 Identification of selected antigen by LC-MALDI Protein immunopurifications were precipitated in ice-cold acetone overnight and resuspended in 40 L of NH 4 HCO 3 50 mM, urea 2 M solution, and digested with 5 L of trypsin solution (0.2 g/L) overnight at 37C

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Consequently, developing new sonosensitizers with enhanced sonosensitizing activity remains a significant challenge for SDT technology

He discovered that by modifying a painkilling peptide our bodies produce, he had created molecules that could also increase production of growth hormone