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Lower levels of GSH can occur because of nonavailability of LC from food consumption, increased ROS production and oxidative stress from energy-rich diet consumption, and/or increased utilization of GSH relative to its biosynthesis

Unlike replacement of solutions by microperfusion (from 1 mM to 100 mM glutamate), EPSCs recovered with much faster kinetics upon glutamate uncaging with a time constant of ~15 s, which is 10100 times faster than those estimated in isolated vesicles (Naito and Ueda, Figure 3 Despite this first success toward estimating the rate of glutamate transport in living synapses, there remain several critical concerns regarding the protocol

Cell suspensions were then applied to the column, and the flow-through was collected in a new 15-ml tube

And I think that theyre probably probably many of them and possibly most of them are wrong

Mechanisms preceding cell death were assessed by measuring reduced glutathione (GSH) levels, glutathione-related enzymes activities, and apoptosis markers (caspase-8, Bax, Bcl-2, and caspase-3)