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During pyroptosis, proteins such as NLRP3, cleaved-caspase-1, cleaved-IL-1, and cleaved-GSDMD serve as essential molecular markers, with their dynamic changes directly reflecting the activation status and regulatory mechanisms of the pyroptosis pathway [47, 48]

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Unresolved questions include the tissue-specific regulation of ULK1 complexes in PCa autophagy, role of CPS1 in nucleotide metabolism, and spatiotemporal dynamics of ATF6-mediated therapy resistance, all critical for advancing precision therapies in this metabolic-death nexus